A large number of various types of neurons must be generated at the optimal time and location for successful development of the functional brain. The molecular mechanisms underlying production of neuronal diversity has been largely unknown. In neural stem cells, several transcription factors are expressed transiently, which are called Temporal Factors, and they determined types of neurons born. However we do not know how the Temporal Factors specify the neuronal types. In this study, I investigate DNA-binding sites of each Temporal Factor by DamID technique to reveal molecular mechanisms that regulate producing neuronal diversity.