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Associate Professor, Faculty of Environmental, Life, Natural Science and Technology, Okayama University *Profile is at the time of the award.
2024Inamori Research GrantsBiology & Life sciences
We are working to decipher and control genetic information encoded in "amino acid" sequences rather than DNA sequences. One of our goals is to create a super Escherichia coli capable of expressing any protein. If you are interested, please contact us!
To elucidate the function of ABCF proteins, translation factors that facilitate the synthesis of amino acid motifs that are difficult to synthesize, we performed large-scale mutagenesis. When amino acids that interact with specific regions of the translation apparatus were mutated, the translation-enhancing activity was disrupted in some of the four ABCF paralogs but not in others, suggesting that each factor acts on the translation apparatus through a distinct mode of action. These results were consistent with structural analyses of ABCF–ribosome complexes using cryo-electron microscopy.
Yushin Ando, et al. (2025) A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism Nature Communications 16, 2323 https://doi.org/10.1038/s41467-025-57659-z
Tadashi Hamano, et al. (2024) STALL-seq: mRNA-display selection of bacterial and eukaryotic translational arrest sequences from large random-sequence libraries Journal of Biological Chemistry 300, 107978 DOI: 10.1016/j.jbc.2024.107978
Akinao Kobo, et al. (2024) Non-specific N-terminal tetrapeptide insertions disrupt the translation arrest induced by ribosome arresting peptide sequences Journal of Biological Chemistry 300, 107360 DOI: 10.1016/j.jbc.2024.107360
Yuhei Chadani, et al. (2024) The ABCF proteins in Escherichia coli individually cope with ‘hard-to-translate’ nascent peptide sequences Nucleic Acids Research 52, 5825–5840 https://doi.org/10.1093/nar/gkae309
Biology & Life sciences
