Jumpei Taguchi

Project Assistant Professor, The Institute of Medical Science, The University of Tokyo *Profile is at the time of the award.

2024Inamori Research GrantsBiology & Life sciences

researchmap

Research topics: BAC-based humanization with CRISPR-Cas9 ribonucleoprotein in mouse embryonic stem cells

Keyword

Summary: Mice have a genome that is 99% similar to humans and are useful model animals for diverse range of medical and life science researches. On the other hands, since there are some aspects that differ from humans, such as control mechanisms of gene expression and three-dimensional structure of proteins, it has been pointed out that there is a need for genomic humanization technology to replace target mouse gene locus with human gene locus. However, genomic humanization requires inducing recombination of a wide range of genomic regions, and versatile genomic humanization technology has not been established. In this research, we aim to develop full-length genomic humanization technology targeting genomic regions over 100kb by focusing on ES cells that has high homologous recombination efficiency.

Message

I'm great honored to receive generous support for my research as Inamori Research Grants. I would like to develop a versatile genomic humanization technology and contribute to advancement of medical and life science research.

Outline of Research Achievments

This study was conducted with the aim of developing a fundamental technology for the versatile generation of full-length gene humanized mice. The applicant devised a method using two-step genome editing for ES cells, and succeeded in developing full-length gene humanization technique targeting a 200 kbp length, which covers 93% of all human genes. In addition, this method induced humanization with an efficiency of about 10-15% at all gene loci tested, which is more than 50 times higher than previous reports.

Find other recipients

Biology & Life sciences

Takanori Wakatake

Nara Institute of Science and Technology

Hirohisa Ohno

Kyoto University

Tomonori Nakamura

Kyoto University

Yukiko Kimura

National Institutes of Natural Sciences

Akihiro Funamizu

Information Science

Yohei Hayashi

Tohoku University

Yuki Honda

Material Science

Hiroshi Miyanishi

University of Miyazaki

Daisuke Ono

Biology & Life sciences Life Science

Miyako Shiraishi

Osaka University

Makoto Kobayashi

Ryusuke Hatae

Kyushu University

Tomonori Matsumoto

Biology & Life sciences Medicine

Keita Takahashi

Kana Furukawa

Osaka University

Atsushi Anzai

Biology & Life sciences Medicine

Takahito Iga

Keio University

Miharu Maeda

Biology & Life sciences Life Science

Shun Shibata

Tohoku University

June-Sik Kim

RIKEN

Shizue Ohsawa

Nagoya University

Toshiki Sawada

Material Science

Masafumi Kodama

Biology & Life sciences

Satoshi Miura

Information Science

Akiko Kanehara

Kunio Kondoh

Toshiyuki Kowada

Biology & Life sciences Life Science

Masashi Mamada

Material Science

Tomohisa Hosokawa

Nagoya University

Atsushi Fujimura

Okayama University

Nami Minato

Chihiro Maeda

Material Science

Shu Nakao

Ritsumeikan University

Yuta Hiraike

The University of Tokyo

Takumi Suzuki

Ibaraki University

Mizuki Endo

Nobuharu Suzuki

Tokyo Medical and Dental University

Daisuke Takimoto

Material Science

Masayuki Morita

Ehime University

Yusuke Kimata

Biology & Life sciences Life Science

Misaki Ishibashi

Kyoto University

Shunsuke Kon

Tokyo University of Science

Tsuyoshi Waku

Doshisha University

Ritsuko Morita

Biology & Life sciences Life Science

Akihiro Nishiguchi

National Institute for Materials Science

Kaori Sako

Tetsuya Takano

Kyushu University

Akihiro Goto

Biology & Life sciences Life Science

Asano Ishikawa

Research Organization of Information and Systems

Tetsuya Kadonosono

Tokyo Institute of Technology

Koji Atarashi

Taro Fuchikawa

Osaka City University

Takumi Ogawa

Osaka Prefecture University

Teruhiko Imamura

Hiroshi Nishimasu

The University of Tokyo

InaRIS Fellow

Takaaki Ozawa

Osaka University

Ryo Fujimoto

Kobe University

Kenta Hirami

Tomomi Koshiyama

Ritsumeikan University

Tomomi Tsunematsu

Hokkaido University

Hideaki Morishita

Biology & Life sciences Life Science

Kenta Maruyama

Aichi Medical University

Hidehisa Kawashima

University of Tsukuba

Takashi Iwasaki

Tottori University

Chikara Abe

Gifu University

Kenji Ikeda

Tokyo Medical and Dental University

Hiroshi Suzuki

Nagoya University

InaRIS Fellow

Susumu Jitsuki

Biology & Life sciences Life Science

Yuichi Yagita

Kyushu University

Miu Tamamitsu

The University of Tokyo

Hiroshi Nomoto

Masanori Nomoto

University of Toyama

Masaki Uchida

Tsuyoshi Hirashima

Kyoto University

Masayuki Tera

Tokyo University of Agriculture and Technology

Hironori Takeda

Hiroshima University

Kiyoharu Fukushima

Kyoto University

Yuka Iwasaki

RIKEN

Gosuke Hayashi

Pharmacy Biology & Life sciences

Akari Fujimoto

Takuya Motooka

Ryohei Thomas Nakano

Hokkaido University

Nobuhiko Yokoshi

Keio University

Toyoaki Natsume

Research Organization of Information and Systems

Takashi Tsuchimatsu

Chiba University

Hideaki Kato

The University of Tokyo

Miho Shiratori

Juntendo University

Yoshifumi Yamaguchi

Hokkaido University

InaRIS Fellow

Dana Ulanova

Kochi University

Ayako Gotoh

Konan University

Masahiro Morimoto

Yuya Sanaki

University of Tsukuba

Tomoya Yamaguchi

Kumamoto University

Satoru Morimoto

Keio University

Takahiro Masuda

Yu Miyamoto

Osaka University

Hiroyoshi Y. Tanaka

Okayama University

Yuto Takenaka

Ritsumeikan University

Hidefumi Shinohara

Nagoya University

Hirofumi Toda

University of Tsukuba

Kazuya Matsuo

Tomoko Okamoto

Yuhei Chadani

Okayama University

Shintaro Yamaguchi

Masayuki Tsukasaki

The University of Tokyo

Junichi Kikuta

University of Tsukuba

Yu Fukasawa

Tohoku University

Takuma Kokusho

Koichi Murata

Kyoto University

Michihito Kono

So Nagaoka

Biology & Life sciences Life Science

Masahiko Saito

Material Science

Akiko Ogawa

Biology & Life sciences Life Science

Kenji Osabe

Tokyo Institute of Technology

Kuniyasu Saitoh

Hiroshi Masai

Material Science

Shohei Nosaki

University of Tsukuba

Masashi Kanayama

Tokyo Medical and Dental University

Jun-ichi Sakamaki

Juntendo University

Atsushi Saito

Sapporo Medical University

Taichi Noda

The University of Tokyo

Kazuhiko Yamamuro

Seiji Takashima

Shinshu University

Yusuke Kobayashi

Hisaaki Hirose

Osaka University

Yoshiaki Abe

University of Tsukuba

Tomoyuki Furuta

Okayama University

Keiichiro Suzuki

Osaka University

Masaru Hojo

Kwansei Gakuin University

Seiya Takayanagi

Wakana Tanaka

The University of Tokyo

Mizuki Watanabe

Hokkaido University

Masaki Takasugi

Osaka Metropolitan University

Yuma Takahashi

Nara Institute of Science and Technology

Aiko Sada

Okayama University

Kyogo Kobayashi

Kyushu University

Tomotaka Ishizaki

Nagoya University

Takayuki Kuraishi

Kanazawa University

Takashi Shimada

Biology & Life sciences

Yasuo Oguri

Biology & Life sciences Life Science

Kotaro Mizuta

Kyoto University

Koki Katsuhara

Okayama University

Kazutoshi Kasho

Biology & Life sciences Life Science

Kazutoshi Iijima

Yokohama National University

Yuichiro Mishima

Kyoto Sangyo University

Tomomi Wakabayashi

Osaka University

Kazunori Kume

Hitoshi Miyakawa

Utsunomiya University

Kenichi Watanabe

Masato Yamamichi

Research Organization of Information and Systems

Takashi Tanaka

Yosuke Togashi

Biology & Life sciences Medicine

Kozue Shiomi

Biology & Life sciences

Eiji Miyauchi

Gunma University

Yoshihiro Ujihara

Nagoya Institute of Technology

Yoshiyuki Manabe

Tokai University

Noriyoshi Usui

Osaka University

Masakazu Hirota

Takeshi Kawabe

Biology & Life sciences Medicine

Tomohiro Otsuka

Shiho Makino

The University of Tokyo

Shintaro Munemasa

Okayama University

Minako Ito

Tsuyoshi Terakawa

Kyoto University

Shohei Yamamoto

Tokyo Medical and Dental University

Kyoko Miura

Kumamoto University

Yusaku Iwasaki

Mitsuru Tsuge

Okayama University

Hidenori Nishio

Nagoya City University

Katsuma Hayashi

Kyoto University

Arisa Hirano

University of Tsukuba

Taichi Itoh

Biology & Life sciences

Ryo Ichijo

Kyoto University

Hidenori Okamura

Tohoku University

Azusa Kondoh

Pharmacy Biology & Life sciences

Katsuyoshi Takaoka

Takashi Fukaya

The University of Tokyo

Akihiko Mougi

Shimane University

Misuzu Hashimoto

Gifu University

Keisuke Ikawa

Nagoya University

Takayuki Suzuki

Nagoya University

Satoshi Iwakami

Kyoto University

Tadayoshi Karasawa

Jichi Medical University

Masayuki Sakamoto

Kumamoto University

Mitsuaki Kashiwagi

Biology & Life sciences Life Science

Kenichi Kimura

University of Tokyo

Rie Wakabayashi

Kyushu University

Ayaka Ito

Satoshi Minami

Osaka University

Kazuki Ide

Kunihiko Morihiro

The University of Tokyo

Saori Morino-Koga

The University of Tokyo

Ayuko Hoshino

The University of Tokyo

InaRIS Fellow

Yuji Sakai

Tokushima University

Takuya Matsumoto

Osaka Prefecture University

Toshiro Moroishi

Masayuki Munekane

Kanazawa University

Yoshihiro Sakita

Shunsuke Tagami

RIKEN

Daisuke Ino

Osaka University

Yuichiro Nakata

Chiba University

Chie Aoki-Utsubo

Kobe University

Yukiko Kamiya