Masayuki Sakamoto

Associate Professor, Graduate School of Biostudies, Kyoto University *Profile is at the time of the award.

2023Inamori Research GrantsBiology & Life sciences

website researchmap

Research topics: Rational design of ultra-bright probe for deep brain imaging

Keyword

Summary: Measuring neural activity using genetically encoded fluorescent calcium indicators is widely used to elucidate the circuit mechanisms of higher brain functions. However, because existing calcium sensors have insufficient brightness, calcium imaging has been limited to the superficial areas in vivo. In this research project, I will develop a new calcium sensor with ultra-high brightness that enables non-invasive imaging of deep brain regions.

Comment

Thank you very much for accepting my application for the Inamori research grant. I hope to contribute to progress in science by developing excellent sensors.

Outline of Research Achievments

We developed a new red fluorescent calcium sensor, RCaMP3, suitable for in vivo imaging. To improve its performance for in vivo two-photon imaging, we optimized its fluorescence properties and expression level, resulting in increased brightness and sensitivity. As a result, we successfully performed in vivo mesoscale imaging with single-cell resolution, which had been difficult to achieve with conventional probes.

Yokoyama T, Manita S et al. (2024) A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nature Methods 21, 897-907 doi: 10.1038/s41592-024-02222-9

Find other recipients

Biology & Life sciences

Shunsuke Kon

Tokyo University of Science

Tomoya Yamaguchi

Kumamoto University

Akiko Kanehara

Daisuke Takimoto

Material Science

Takashi Iwasaki

Tottori University

Masahiko Saito

Material Science

Yuichiro Mishima

Kyoto Sangyo University

Seiya Takayanagi

Takahiro Masuda

Mitsuru Tsuge

Okayama University

Takuya Motooka

Tomonori Nakamura

Kyoto University

Tomomi Tsunematsu

Hokkaido University

Keita Takahashi

Tomomi Koshiyama

Ritsumeikan University

Takashi Shimada

Biology & Life sciences

Masahiro Morimoto

Koichi Murata

Kyoto University

Mitsuaki Kashiwagi

Biology & Life sciences Life Science

Masaki Takasugi

Osaka Metropolitan University

Kazuhiko Yamamuro

Hisaaki Hirose

Osaka University

Shun Shibata

Tohoku University

June-Sik Kim

RIKEN

Noriyoshi Usui

Osaka University

Takuma Kokusho

Katsuyoshi Takaoka

Yoshifumi Yamaguchi

Hokkaido University

InaRIS Fellow

Eiji Miyauchi

Gunma University

Hideaki Kato

The University of Tokyo

Masato Yamamichi

Research Organization of Information and Systems

Ryusuke Hatae

Kyushu University

Kunio Kondoh

Tsuyoshi Hirashima

Kyoto University

Keiichiro Suzuki

Osaka University

Yuka Iwasaki

RIKEN

Toshiki Sawada

Material Science

Kazutoshi Kasho

Biology & Life sciences Life Science

Kana Furukawa

Osaka University

Takayuki Suzuki

Nagoya University

Wakana Tanaka

The University of Tokyo

Tomotaka Ishizaki

Nagoya University

Hidenori Nishio

Nagoya City University

Toshiro Moroishi

Hidehisa Kawashima

University of Tsukuba

Rie Wakabayashi

Kyushu University

Akihiro Nishiguchi

National Institute for Materials Science

Takashi Fukaya

The University of Tokyo

Shintaro Munemasa

Okayama University

Mizuki Endo

Akihiro Goto

Biology & Life sciences Life Science

Tetsuya Kadonosono

Tokyo Institute of Technology

Masayuki Tera

Tokyo University of Agriculture and Technology

Satoshi Miura

Information Science

Yuichiro Nakata

Chiba University

Dana Ulanova

Kochi University

Satoshi Minami

Osaka University

Kenichi Watanabe

Satoshi Iwakami

Kyoto University

Yusuke Kobayashi

Yusuke Kimata

Biology & Life sciences Life Science

Chikara Abe

Gifu University

Susumu Jitsuki

Biology & Life sciences Life Science

Yuya Sanaki

University of Tsukuba

Atsushi Anzai

Biology & Life sciences Medicine

Masanori Nomoto

University of Toyama

Tomohiro Otsuka

Kenta Hirami

Miu Tamamitsu

The University of Tokyo

Miyako Shiraishi

Osaka University

Ayako Gotoh

Konan University

Gosuke Hayashi

Pharmacy Biology & Life sciences

Yoshihiro Ujihara

Nagoya Institute of Technology

Takaaki Ozawa

Osaka University

Takumi Ogawa

Osaka Prefecture University

Kazutoshi Iijima

Yokohama National University

Takuya Matsumoto

Osaka Prefecture University

Masaru Hojo

Kwansei Gakuin University

Atsushi Fujimura

Okayama University

Ryo Ichijo

Kyoto University

Yuki Honda

Material Science

Yukiko Kimura

National Institutes of Natural Sciences

Seiji Takashima

Shinshu University

Koji Atarashi

Daisuke Ono

Biology & Life sciences Life Science

Keisuke Ikawa

Nagoya University

Takashi Tsuchimatsu

Chiba University

Misaki Ishibashi

Kyoto University

Hiroshi Nishimasu

The University of Tokyo

InaRIS Fellow

Shu Nakao

Ritsumeikan University

Aiko Sada

Okayama University

So Nagaoka

Biology & Life sciences Life Science

Yuta Hiraike

The University of Tokyo

Yuma Takahashi

Nara Institute of Science and Technology

Nobuhiko Yokoshi

Keio University

Ayaka Ito

Hiroshi Suzuki

Nagoya University

InaRIS Fellow

Tomomi Wakabayashi

Osaka University

Katsuma Hayashi

Kyoto University

Akihiko Mougi

Shimane University

Daisuke Ino

Osaka University

Hideaki Morishita

Biology & Life sciences Life Science

Kyoko Miura

Kumamoto University

Taro Fuchikawa

Osaka City University

Tomonori Matsumoto

Biology & Life sciences Medicine

Tetsuya Takano

Kyushu University

Akiko Ogawa

Biology & Life sciences Life Science

Yoshiaki Abe

University of Tsukuba

Kazuki Ide

Masafumi Kodama

Biology & Life sciences

Yoshihiro Sakita

Hidefumi Shinohara

Nagoya University

Hiroshi Miyanishi

University of Miyazaki

Kaori Sako

Taichi Noda

The University of Tokyo

Yuhei Chadani

Okayama University

Yasuo Oguri

Biology & Life sciences Life Science

Hiroshi Masai

Material Science

Koki Katsuhara

Okayama University

Yuichi Yagita

Kyushu University

Jumpei Taguchi

The University of Tokyo

Toshiyuki Kowada

Biology & Life sciences Life Science

Kyogo Kobayashi

Kyushu University

Kazuya Matsuo

Minako Ito

Toyoaki Natsume

Research Organization of Information and Systems

Kenji Osabe

Tokyo Institute of Technology

Akihiro Funamizu

Information Science

Masayuki Munekane

Kanazawa University

Saori Morino-Koga

The University of Tokyo

Arisa Hirano

University of Tsukuba

Yuto Takenaka

Ritsumeikan University

Takumi Suzuki

Ibaraki University

Azusa Kondoh

Pharmacy Biology & Life sciences

Hiroyoshi Y. Tanaka

Okayama University

Asano Ishikawa

Research Organization of Information and Systems

Ryohei Thomas Nakano

Hokkaido University

Hidenori Okamura

Tohoku University

Hirohisa Ohno

Kyoto University

Miharu Maeda

Biology & Life sciences Life Science

Satoru Morimoto

Keio University

Tsuyoshi Terakawa

Kyoto University

Takayuki Kuraishi

Kanazawa University

Masayuki Morita

Ehime University

Kenta Maruyama

Aichi Medical University

Yosuke Togashi

Biology & Life sciences Medicine

Shunsuke Tagami

RIKEN

Makoto Kobayashi

Junichi Kikuta

University of Tsukuba

Hirofumi Toda

University of Tsukuba

Teruhiko Imamura

Masashi Kanayama

Tokyo Medical and Dental University

Shintaro Yamaguchi

Misuzu Hashimoto

Gifu University

Nobuharu Suzuki

Tokyo Medical and Dental University

Kenichi Kimura

University of Tokyo

Nami Minato

Tomoko Okamoto

Yukiko Kamiya

Yohei Hayashi

Tohoku University

Yu Miyamoto

Osaka University

Atsushi Saito

Sapporo Medical University

Tadayoshi Karasawa

Jichi Medical University

Tomohisa Hosokawa

Nagoya University

Shohei Yamamoto

Tokyo Medical and Dental University

Masashi Mamada

Material Science

Kozue Shiomi

Biology & Life sciences

Yusaku Iwasaki

Kiyoharu Fukushima

Kyoto University

Hiroshi Nomoto

Taichi Itoh

Biology & Life sciences

Yuji Sakai

Tokushima University

Yoshiyuki Manabe

Tokai University

Kazunori Kume

Takahito Iga

Keio University

Takanori Wakatake

Nara Institute of Science and Technology

Ayuko Hoshino

The University of Tokyo

InaRIS Fellow

Tomoyuki Furuta

Okayama University

Masayuki Tsukasaki

The University of Tokyo

Kuniyasu Saitoh

Hironori Takeda

Hiroshima University

Chie Aoki-Utsubo

Kobe University

Kotaro Mizuta

Kyoto University

Shizue Ohsawa

Nagoya University

Ritsuko Morita

Biology & Life sciences Life Science

Akari Fujimoto

Kunihiko Morihiro

The University of Tokyo

Shiho Makino

The University of Tokyo

Chihiro Maeda

Material Science

Tsuyoshi Waku

Doshisha University

Mizuki Watanabe

Hokkaido University

Michihito Kono

Masaki Uchida

Kenji Ikeda

Tokyo Medical and Dental University

Yu Fukasawa

Tohoku University

Takeshi Kawabe

Biology & Life sciences Medicine

Jun-ichi Sakamaki

Juntendo University

Hitoshi Miyakawa

Utsunomiya University

Shohei Nosaki

University of Tsukuba

Miho Shiratori

Juntendo University

Takashi Tanaka

Ryo Fujimoto

Kobe University

Masakazu Hirota